Table 3.

BLAST scores of added reactions in S. cerevisiae

Reactions	E Value	Reaction in S. cerevisiae	Organism
methionine γ-lyase	1e-58,1e-68	Cystathionine gamma-lyase	Pseudomonas putida
2-hydroxyglutarate synthase(no gene identified yet)	----------	---------------	E. coli
2-oxoglutarate reductase/phosphoglycerate dehydrogenase	2e-14, 3e-18	3-phosphoglycerate dehydrogenase	Bacillus subtilis
deoxyribose-phosphate aldolase	7.7, 0.006	Phosphatidylinositol 3-kinase TOR2	Mycoplasma pneumoniae
D-lactate dehydrogenase	2, 3e-04	Protein of unknown function	E. coli
L-tryptophan aminotransferase	no information	no information	Enterobacter cloacae
Methylglyoxal synthase	1.5, 0.001	carbamyl phosphate synthetase	E. coli
phenylpyruvate decarboxylase	3E-35, 1e-39	Chain A, Pyruvate Decarboyxlase	Azoarcus sp. EbN1
tagatose-1,6-bisphosphate aldolase 2	1e-12, 1e-16	Fructose 1,6-bisphosphate aldolase	E. coli
tryptophan 2-monooxygenase	2.2, 5e-05	orf:PZA105	E. coli
5,10-methylenetetrahydrofolate reductase(present in the cytosol)	1E-38, 2e-42	Isozyme of methylenetetrahydrofolate reductase	E. coli
adenine phosphoribosyltransferase(present in cytosol)	2E-25, 1e-29	Adenine phosphoribosyltransferase	E. coli
guanylate kinase(present in cytosol)	9E-38, 7e-42	Guanylate kinase	E. coli
putative NAD+ kinase(present in cytosol)	2E-27, 6e-28	ATP-NADH kinase	E. coli
adenylate kinase(present in cytosol and mitochondria)	7E-51, 5e-55	Adenylate kinase	E. coli

Candidate reactions from the MetaCyc database (along with BLAST scores) added to enable production of no-production metabolites in S. cerevisiae.