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. Author manuscript; available in PMC: 2007 Jul 26.
Published in final edited form as: Mol Cell Endocrinol. 2007 Jan 16;267(1-2):106–115. doi: 10.1016/j.mce.2007.01.001

Fig. 3.

Fig. 3

ERα limits DNase I cleavage at three ERE half sites in the ninth intron of the BARD1 gene. Noncoding strand primers were 32P-labeled and used to PCR amplify a 204 bp (A) or 304 bp (B) region of DNA. Labeled DNA was incubated with 0, 25, 100, 250, or 500 fmol of purified, baculovirus-expressed ERα (lanes 2–6) and exposed to DNase I. DNA that had been cleaved at each G residue was included for reference (lanes 1). Hypersensitive sites are indicated by an asterisk.