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(A) Diagram showing Renilla luciferase reporter constructs, in which the Renilla Kozak sequence is replaced by the 5′-UTR/Kozak regions from human PIPSL (1), chimp PIPSL (2), and PIP5K1A (3). (B) Average amounts of Renilla produced by in vitro transcription/translation reactions using the three constructs ±SD. For each construct, eight replicates were performed using six to eight independent DNA preparations. Human PIPSL translation was significantly reduced (P < 0.001).
