Figure 2.

MGDG synthase expressed in E. coli. (a) Construction of plasmid with GST fusion expression vector of pGEX-3X to generate the mature region of MGDG synthase as a fusion protein with GST. (b) MGDG synthase activity measured by the formation of [³H]MGDG by cell-free extract of E. coli XL1-Blue transformed with pGEX-3X alone or pGEX-3X harboring the cDNA clone. We designated the expression vector pGEX-3X harboring the cDNA clone as pGEX-GT. GEX-3X and 1b-2 indicate E. coli XL1-Blue transformed with pGEX-3X and pGEX-GT, respectively. Plus (+) and minus (−) indicate the crude extracts from XL1-Blue grown in the presence or absence of IPTG, respectively.