Figure 3.

TLC analysis of galactosylation products produced by the expressed fusion protein of MGDG synthase and GST in E. coli extracts. Lane 1, total lipids extracted from spinach, which were stained with anthrone–sulfuric acid; lanes 2 and 3, the reaction products of the extract from GEX-3X, which are untreated and treated with IPTG (1 mM final concentration), respectively; lanes 4 and 5, the reaction products of the extract from 1b-2, which are untreated and treated with IPTG, respectively; and lane 6, the reaction products of MGDG synthase purified from cucumber cotyledons. SQDG, sulfoquinovosyldiacylglycerol.