Figure 1.

Domains of L1 repressor. Chymotrypsin (A) and trypsin (B) digested His-CI fragments were analyzed by Tris-Tricine SDS-16.5% PAGE followed by silver staining. Molecular masses (in kDa) of marker proteins are shown at the left side of gel. 'Chy' and 'Try' indicate chymotrypsin and trypsin, respectively whereas, a – h indicate intact repressor, different digested fragments of repressor, respectively. N-terminal ends of fragments c and h were sequenced. (C) Western blotting analysis of chymotrypsin/trypsin digested His-CI fragments from 2 and 30 mins incubations by a standard procedure as indicated in Materials and method. (D) Summary of proteolysis. The putative domains of CI and its amino acid residues involved in formation of hinge, NTD and CTD are indicated.