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. Author manuscript; available in PMC: 2007 Jul 30.
Published in final edited form as: Traffic. 2006 Aug;7(8):978–992. doi: 10.1111/j.1600-0854.2006.00449.x

Figure 7.

Figure 7

Viral RNA distribution in HeLa and SIRC cells. Cells were infected with the same dose of HIV vector, incubated 2 h at 4 °C and then 4 h at 37 °C. Infected cells were subjected to a stepwise fractionation procedure as described in Figure 5A in RNAse-free conditions. Total RNA was extracted, and samples were treated with RNAse-free DNAse I. After re-purification of the RNA, reverse transcriptase was used to perform first-strand cDNA synthesis. Control reactions were incubated in parallel in the absence of reverse transcriptase. Viral RNA copy number was measured by real-time PCR. Bars represent the mean ± standard deviation of triplicate experiments. Hy, hypotonic buffer; Iso, isotonic buffer; HS, high salt buffer; 1%T, 1% Triton-X buffer; SDS, SDS buffer.