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. 1996 Nov 26;93(24):13565–13570. doi: 10.1073/pnas.93.24.13565

Figure 3.

Figure 3

Structure of pRP1001 and vector amplification in AdLC8-infected 293Cre4 cells. (A) pRP1001 (shown in linear form), a derivative of pFG140 (35), contains sequences corresponding to the left end (3–5789 bp interrupted by the insertion of a bacterial plasmid [pMX2] at 1338 bp), and right end (29,792–35,924 bp) of the Ad genome, including the Ad packaging signal (ψ) and inverted terminal repeats. pRP1001 also contains the E. coli β-galactosidase gene (lacZ) between the murine CMV immediate early promoter and the simian virus 40 polyadenylyation signal, and an 8.3-kb insert of lambda DNA. In the presence of AdLC8 in 293 cells, pRP1001 is converted into linear molecules, replicated, and packaged into infectious virions (AdRP1001). (B) Serial passage of AdRP1001 (▪) in AdLC8-infected 293Cre4 cells. The titers on 293 cells (□) and A549 cells (░⃞) are also shown for each passage.