Figure 6.

Blood vessels persist in the wounds or mice lacking CXCR3. Neovascularization in CXCR3^−/− mice was assessed using immunostaining of von Willebrand factor antigen outlining blood vessels of the mice. A: Quantitation of the number of capillaries, as determined by von Willebrand factor staining and morphometry, with a low-power field in the center of the wound is shown in the graph. WT mice are represented by filled diamonds with solid line, and CXCR3^−/− are represented by open diamonds with dashed line. This was derived from two independent experiments of three mice in each group/time period (n = 6) with each mouse evaluated in three random low-power fields (shown are mean ± SD, *P < 0.05). B: Representative von Willebrand factor immunostaining demonstrates the paucity of capillaries (arrows) at day 90 in wild-type wound compared with the CXCR3^−/− wounds. The images measure 300 μm on each side. Original magnifications, ×400.