Figure 3.

I/R induces dynamic phosphorylation of FAK, paxillin, and Src. Rats were subjected to 30 minutes of ischemia followed by reperfusion for 0 to 24 hours. Frozen sections (10 μm) were stained for FAK (green) and P_Y397-FAK (red) to determine differential tyrosine phosphorylation and colocalization (yellow) at FAs (A). In addition, frozen sections were prepared for Western blot analysis and stained for P_Y397-FAK and FAK (B), P_Y118-paxillin and paxillin (D), and P_Y576-FAK, P_Y861-FAK P_Y416-Src kinase, and P_Ser17/221-ERK (C). All sections were imaged using a confocal laser scanning microscope. Data are representative of proximal tubules in three different rats.