C3b and MAC deposition in the kidney at 24 hours after PsTX-T administration. Twenty-four hours after PsTX-T injection, massive C3b deposition was found in the glomeruli and tubular epithelium (A, E, I, and M) and strong MAC deposition was present in glomeruli and tubular epithelium (C, G, K, and O). Vehicle controls are represented in B, D, F, H, J, L, N, and P. The tissue locations are shown on the left side. Legends at the top show what was detected and what was injected. Original magnifications: ×400 (A–D); ×200 (E–P).