CReg expression after ex vivo exposure of kidney sections to PsTX-T. Sections were stained for CD55 (A–C), Crry (D–F), or CD59 (G–I). Expression of each of the CRegs was decreased in glomeruli following PsTX-T treatment (A, D, and G). Tubular expression of Crry and CD59 were preserved. In contrast, all CRegs were preserved when sections were incubated with PsTX-T mixed with protease inhibitor cocktail (B, E, and H) compared with buffer only controls (C, F, and I). Original magnification, ×400.