Figure 4.

Systemic administration of DX109 suppresses EAU. A: Eyes were harvested at the days postimmunization indicated, and average disease score ± SD of inflammatory infiltrate and structural disease is shown. n = 6/time point. Data representative of two independent experiments n = 6. Mann-Whitney U-test was performed total disease score. *P = 0.0075, ⁺P = 0.0043. B: Representative 12-μm sections from two separate experiments at days 14, 21, and 28 after immunization. Photographs show sections stained with anti-CD45 antibody counterstained with hematoxylin, from mice either treated with an isotype control or DX109 administered on days 7, 14, and 18 after immunization. In isotype mAb-treated animals, there was marked retinal destruction as indicated by arrows, with retinal detachment (1), chronic retinal folding (2), and retinal vasculitis (3). In the majority of DX109-treated animals, the retina appeared normal with maintained photoreceptors (4), although by day 28, retinal folds and low-level infiltrate were present. Original magnifications, ×100.