Table 1.
NO Production from Retinal-Derived Macrophages
| Day | WT
|
CD200−/−
|
||
|---|---|---|---|---|
| Control | IFNγ | Control | IFNγ | |
| 15 | 1028 (±500) | 1568 (±209) | 2271 (±215) | 2597 (±452) |
| 25 | 1280 (±116) | 2558 (±558) | 1542 (±193) | 1509 (±711) |
Eyes from WT and CD200−/− animals were harvested at days 15 and 25 after immunization, and F4/80-positive macrophages were isolated. Cells were plated at 5 × 105 cells/ml; after 2 hours, media alone or recombinant IFNγ were added to give final concentration of 20 U/ml. After 24 hours, NO production (nM) in culture supernatants was analyzed using the Griess reaction. Results are calculated as mean ± SEM of eight wells.