Figure 9.

Effect of angiotensin II type 1 receptor blockade on the urinary AER. Four groups of mice were studied: Ace2^+/yIns2^WT/C96Y mice and Ace2^−/yIns2^WT/C96Y, either untreated or treated with the angiotensin II type 1 receptor blocker irbesartan (IRB) from weaning until 3 months of age (n = 6 in each group). The urinary AER was measured with an enzyme-linked immunosorbent assay in 24-hour urine samples. The AER was greater in untreated Ace2^−/yIns2^WT/C96Y mice compared with untreated Ace2^+/yIns2^WT/C96Y mice but this difference was prevented by treatment with IRB. Data are presented as mean ± SEM. **P < 0.05 compared with Ace2^+/yIns2^WT/C96Y mice, ^§P < 0.05 compared with IRB Ace2^+/yIns2^WT/C96Y mice, and ^‡P < 0.05 compared with Ace2^−/yIns2^WT/C96Y mice.