Figure 2. Analysis of signaling pathways involved in OxPAPC-induced cytoskeletal remodeling.
EC grown on glass coverslips were preincubated with genistein (100 μM), small GTPase inhibitor toxin B (20 ng/ml), PKA inhibitor (20 μM), PKC inhibitor (20 μM), Rho kinase inhibitor Y27632 (5 μM), MEK/Erk1,2 inhibitor U0125 (5 μM), p38 MAP kinase inhibitor SB203580 (20 μM), or PI3-kinase inhibitor LY294002 (25 μM) for 30 min followed by addition of OxPAPC (20 μg/ml) for 30 min. Cytoskeletal remodeling was assessed by immunofluorescent staining for F-actin with Texas Red phalloidin. Results are representative of three independent experiments.