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. 2007 Aug 1;117(8):2133–2144. doi: 10.1172/JCI31807

Figure 1. High oxidative stress in Foxo3–/– erythrocytes.

Figure 1

(A) Kaplan Meier survival curve of wild-type and Foxo3–/– mice (n = 6 in each group) treated with a single dose of intraperitoneal injection of phenylhydrazine (PHZ; 100 mg/kg). P < 0.0001, log-rank test; 1 of 2 independent experiments is shown. (B) ROS measurement in WT and Foxo3–/– erythrocytes (note absence of any exogenously added peroxide). (C) Histogram of results from B (mean ± SEM, n = 6; **P < 0.02). (D) Protein oxidation in erythrocytes. DNPH, 2,4-dinitrophenylhydrazine. (E) Quantitative analysis of the oxidative status of WT (n = 3) and Foxo3–/– (n = 4) erythrocytes by comparison of signal intensity of lanes in D using BandScan software version 4.5 (Glyco). *P < 0.05, Student’s t test.