Figure 2. Reduced erythrocyte lifespan in Foxo3^–/– mice.

(A) Erythrocyte lifespan was measured by in vivo biotin labeling. At day 0, 95% of erythrocytes were labeled. Animals were bled every 5 days, and the surviving labeled cells were analyzed by flow cytometry. (B) Reticulocyte index was measured in Foxo3^–/– and WT mice. Results represent mean ± SEM; n = 6 in each group. *P < 0.05, **P < 0.01, Student’s t test.