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. 2007 Aug 1;117(8):2095–2104. doi: 10.1172/JCI32022

Figure 2. Induction of cytoplasmic CatL protein and activity by LPS.

Figure 2

(A) Cultured podocytes were stained using anti–LAMP-2 antibodies and the BIOMOL CV-CatL/B activity detection kit. Control cells, cells treated with 50 μg/ml of LPS for 24 hours, and cells treated with LPS and 1 μM of the selective CatL inhibitor Z-FF-FMK are shown. (B) Labeling of cultured podocytes using anti-CatL antibody, untreated or after treatment with 50 μg/ml of LPS for 24 hours. (C) Labeling of cultured podocytes using anti-CatB antibody, untreated or after treatment with 50 μg/ml of LPS for 24 hours. (D) Schematic of CatL mRNA and resulting proteins. (E) Subcellular fractionation of podocytes in isotonic sucrose prior to and 24 hours after LPS treatment. Total proteins from the soluble (S) and the particulate (P) fractions were analyzed by Western blotting.