Fig. 2.

Treatment of TRAIL proteins enhances FHIT-mediated apoptosis. The NSCLC cell lines A549 and H1299 were transduced with Ad-FHIT and treated with a recombinant TRAIL protein (10 ng/ml). On days 2 and 4 after transduction, cells were harvested, and apoptosis was examined using TUNEL-based FACS analysis. PBS, an empty vector (EV), and LacZ were used as negative controls, and Ad-p53 was used as a positive control. Apoptosis is shown as the relative percentage of apoptotic cells. Three separate experiments were performed in duplicate and each bar denotes mean ± SEM of three experiments. P + F means p53 + FHIT.