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. Author manuscript; available in PMC: 2008 Jul 13.
Published in final edited form as: Biochem Biophys Res Commun. 2007 May 15;358(4):1076–1079. doi: 10.1016/j.bbrc.2007.05.037

Figure 1. Western blot analysis of 293 transformants.

Figure 1

293 cells were transformed with pCMV or pCMV-DBC2 using Lipofectamine Transfection Reagent (Invitrogen, Carlsbad, CA USA) and harvested with CelLytic MT, 48 hours after transformation. T and E in the figure represent pCMV-DBC2 and pCMV transformants, respectively. Screening of cell cycle regulators. Expression levels of CCNE, CCNB, CCND1, CCNH, p34 and p27 were analyzed with corresponding antibodies: H-145, H-433, M-20, C-18, H-297 and C-19 respectively (Santa Cruz Biotechnology).