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. Author manuscript; available in PMC: 2008 Apr 27.
Published in final edited form as: J Mol Biol. 2007 Feb 9;368(2):336–348. doi: 10.1016/j.jmb.2007.02.008

Figure 3.

Figure 3

Relative abundance of size-fractionated nascent T4 DNA synthesized early during infection. Wild type T4D and the recombination uvsX mutant were used to infect E. coli BL21(DE3) cells at a multiplicity of 0.5 viruses per cell. Total viral DNA harvested from infections at 6, 7, and 8 minutes post infection was size fractionated on alkaline agarose gels and used to generate random primed probes as described in Materials and Methods. These labeled DNAs were then used to probe the T4 genomic macroarray, immobilized on nylon membranes, and the relative abundance of viral DNA at each locus along the array was calculated as described in Materials and Methods. (A) Wild type nascent T4D DNA, 3–6 kb. (B) Wild type nascent T4D DNA, 6–10 kb. The filled circles used in (A) and (B) are 6 minutes, black: 7 minutes, orange: 8 minutes, blue. (C) Mutant uvsX nascent DNA, 3–6 kb. (D) Mutant uvsX nascent DNA, 6–10 kb. The open circles used in (C) and (D) are 6 minutes, black: 7 minutes, orange: 8 minutes, blue. The position along the 168 kb T4 genome is identified in kilobases along the x-axis.