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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Blood Cells Mol Dis. 2007 Apr 11;39(1):44–55. doi: 10.1016/j.bcmd.2007.02.006

Figure 2. PFGE structural analysis of three Δ5’HS4 β-YAC transgenic mouse lines.

Figure 2

Structural analysis was performed as described in Materials. A schematic diagram of the YAC is shown at the top of the figure; above that, the diagnostic 145 Kb SfiI fragment encompassing most of the β-globin locus is illustrated. This SfiI fragment is larger than the normal 140 Kb SfiI fragment because the 5’HS4 deletion removes one of the diagnostic SfiI sites, but juxtaposes another upstream of 5’HS5 (see Figure 1). Autoradiographs of lines Δ6, Δ35 and Δ38 are displayed. The probes used are listed above each autoradiograph. The location of the145 Kb SfiI fragment is indicated on the left. All three lines contain two intact copies of the locus. Copy numbers were validated by standard Southern blot analysis.