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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Blood Cells Mol Dis. 2007 Apr 11;39(1):44–55. doi: 10.1016/j.bcmd.2007.02.006

Figure 4. Confirmation of presence of 5’HS5 in Δ5’HS4 lines.

Figure 4

Southern blot hybridization analysis of SfiI digested samples fractionated by PFGE (see Figures 3 and 4) demonstrates linkage of 5’HS5 to the remainder of the β-globin locus in lines Δ6 and Δ35. Although in the Δ38 line 5’HS5 is not contiguous with the β-globin loci, at least 556 bp DNA is present upstream of the 5’HS4 deletion as shown by the correct size EcoRI fragment displayed in Figure 3.