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Gelatin substrate zymography reveals that MMP-9 activity was increased >4-fold in MCTsl/sl lungs compared with MCT+/+ lungs, whereas MMP-2 activity was not different between groups. Note that MMP-2 and MMP-9 are detected as both a higher-molecular-weight proform and a lower-molecular-weight active form. STD indicates recombinant MMP-9.
