Figure 3.

Transient binding of Dnmt1 at replication sites in different S-phase stages. (A) GFP-Dnmt1^wt (green) and RFP-PCNA (magenta) co-expressed in mouse C2C12 cells co-localize at replication sites in early, mid and late S-phase. Fluorescence bleaching of a small region of interest reveals fast recovery of GFP-Dnmt1^wt, whereas RFP-PCNA shows almost no recovery within the observation period. (B) Quantitative evaluation of the FRAP experiments shown in (A) reveal a significantly faster recovery of GFP-Dnmt1^wt in early and mid S-phase compared to late S-phase. Scale bars: 5 µm.