Figure 6.

Immobilization of Dnmt1 does not prevent progression of DNA replication. S-phase C2C12 myoblasts expressing GFP-Dnmt1^wt (green) and RFP-PCNA (magenta) were imaged without drug treatment (upper row) and at the indicated time points after addition of 10 µM 5-aza-dC to the medium (lower row). In the control cell the two constructs largely co-localize during transition from mid to late S-phase, whereas in the presence of 5-aza-dC progressive separation of green and red foci indicate immobilization of GFP-Dnmt1^wt at postreplicative hemimethylated sites and progression of replication foci containing RFP-PCNA. Projections of confocal mid sections are shown. Scale bar: 5 µm.