Figure 2.

(A) Aging protocol for transmission of mtDNA length variants. This culture protocol was followed at both 25°C and 18°C with the mothers used for the two temperature treatments being siblings of the same heteroplasmic female. The data reported in Table 1 were obtained from the offspring generation, and the data reported in Table 2 were obtained from the mothers after they had laid eggs. (B) Aging and mating protocol. This procedure was replicated across different female sublines.