Table 1.

Mitochondrial genotype frequencies in early and late cohorts of F₁ offspring from heteroplasmic D. melanogaster mothers carrying two different length variants of mtDNA

Line-subline	Temp	Early lay; late lay	Offspring, %L ± var	n	Δ%L, Late-early	Per-day, Δ%L	U	P <
4-19D4-D1	25°C	0	0.771 ± 0.0038	14	0.097	0.00691	183	0.001
		15	0.868 ± 0.0040	15
D2	25°C	0	0.911 ± 0.0036	13	−0.053	−0.00355	116	0.1
		15	0.858 ± 0.0074	13
G4	25°C	2	0.922 ± 0.0018	11	0.040	0.00199	129	0.005
		22	0.962 ± 0.0002	14
H4	25°C	2	0.845 ± 0.0031	10	0.098	0.00464	207	0.001
		23	0.944 ± 0.0004	14
B2	18°C	2	0.734 ± 0.0048	15	0.161	0.00845	146	0.001
		21	0.895 ± 0.0067	10
D1F	18°C	2	0.760 ± 0.0114	13	0.068	0.00676	121	0.1
		12	0.828 ± 0.0052	14
D3	18°C	3	0.726 ± 0.0048	15	0.125	0.00889	201	0.001
		17	0.850 ± 0.0058	15
D4	18°C	3	0.796 ± 0.0037	15	0.042	0.00301	116	0.11
		17	0.838 ± 0.0022	12
G2	18°C	2	0.905 ± 0.0072	12	0.058	0.00276	152	0.001
		23	0.963 ± 0.0014	15
H1	18°C	2	0.819 ± 0.0025	15	0.110	0.00733	222	0.001
		17	0.929 ± 0.0012	15
5-27H3-D	25°C	0	0.488 ± 0.0041	15	0.105	0.00955	160	0.004
		11	0.593 ± 0.0090	13
J	25°C	0	0.532 ± 0.0010	10	0.131	0.01191	88	0.001
		11	0.663 ± 0.0031	9
O	25°C	0	0.521 ± 0.0024	12	0.068	0.00618	96	0.02
		11	0.589 ± 0.0038	11
Mothers held as virgins for 10 days before mating
4-19D4-B6	25°C	0	0.839 ± 0.0086	13	0.154	0.01029	195	0.001
		15	0.993 ± 0.0001	13
D5	25°C	0	0.916 ± 0.0037	15	0.042	0.00219	142	0.07
		19	0.958 ± 0.0019	14
B8	18°C	0	0.636 ± 0.0056	12	−0.002	−0.00011	88	0.7
		15	0.635 ± 0.0074	14
G8	18°C	0	0.839 ± 0.0058	14	0.055	0.01363	121	0.1
		4	0.893 ± 0.0112	13
H7	18°C	0	0.880 ± 0.0035	10	0.054	0.00385	98	0.01
		14	0.934 ± 0.0010	12

Line and subline refer to the strain and mother within strain, respectively, of D. melonogaster (see Materials and Methods). Temp. refers to the temperature at which the mothers laid eggs and the eggs developed to emergence of F₁ adult. Early and late lay refer to the age of the heteroplasmic mothers (in days) at the start of a 2-day period of egg collection. Heteroplasmic genotypes are expressed as the mean percent of the long (L) mtDNA among individual flies in a sample of offspring ± the variance (n = sample size of F₁ off spring). Δ%L is the change in frequency of the L-mtDNA between the early and late cohorts. The per-day Δ%L is the value of the Δ%L divided by the difference in age of the mother between the early and late lay dates. The U value is for Mann–Whitney tests between early and late cohorts.