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. 2007 Jun 18;35(13):4359–4368. doi: 10.1093/nar/gkm444

Figure 6.

Figure 6.

(A) Shows the sequence of the YB-1-binding motif inserted in the PstI/KpnI restriction sites of the pES plasmid (pTB YB1PK). (B) Left panel shows a comparison of the levels of CFTR exon 9 inclusion in the TG11T5, pES and pTB YB1PK plasmids following transfection in Hep3B cells. The position of the transcripts including exon 9 (ex9+) and lacking exon 9 (ex9−) are marked on the right. The results of three independent experiments as quantified by radioactive RT–PCR are reported in (B), right panel. The effect of cloning the YB-1 sequence only in the PstI site of the pES plasmid (pTB YB-1P) as opposed to the PstI/KpnI sites (pTB YB-1PK) is shown in (C). The schematic diagram on the left shows the composition of the unique splice product observed in lane 1 whilst the position of the transcripts including exon 9 (ex9+) and lacking exon 9 (ex9−) are marked on the right.