Figure 5.

(A) The sequence of the CFTR intronic region inserted in the SmaI site of PY7 that contains the A > G mutation responsible for creating a high score donor site (5′pe) (highlighted in bold). The positions and scores of the acceptor (3′pe) and additional donor splice site (5′ss) that occur within this sequence are also shown. (B) In vitro splicing reaction of two transcripts, one that did not contain the A to G substitution (CFTR WT) and the other carrying the A to G pseudoexon-creating mutation (CFTR MUT). (C) The structural analysis of CFTR MUT using limited RNAse digestion with the S1, V1 and T1 enzymes (nomenclature of the cleavages and of the enzymes used is as described in Figure 2 legend) and the in silico predicted structure on which the individual cleavages have been reported (D).