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Mapping Rna14p–Rna15p interaction. (a) Pull-down experiments were performed between Rna15p and His-tagged Rna14p deletion mutants. Proteins were revealed with polyclonal antibodies to Rna15p and monoclonal antibodies directed against the His-tag. T, total extract; B, bound fraction. (b) Rna14p deletion constructs used in pull-down experiments. ‘+’ and ‘–’ corresponds to interaction and no interaction, respectively.
