Figure 2.

Sequencing gel northern blot analysis of scRNA processing pattern in single (lanes 2–5), double (lanes 6–8) and quadruple (lane 9) exoribonuclease mutants. The scRNA riboprobe, complementary to the large internal loop, was the probe. Below each lane is the genotype of the strain from which RNA was isolated. Sequencing reaction that serves as size marker is in the left four lanes, with nucleotide sizes indicated on the left. Identity of detected bands is indicated on the right. Schematic diagrams, from top to bottom, represent: full-length scRNA; scRNA cleaved at Bs-RNase III site A only; scRNA cleaved at Bs-RNase III sites A and b; and fully mature scRNA. Full-length and mature scRNA and products from Bs-RNase III cleavage are indicated by arrows; products that are thought to arise by processing after endonuclease cleavage at site X are indicated by lines without arrowheads.