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. 2007 Jun 18;35(13):4464–4473. doi: 10.1093/nar/gkm460

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — (A) Products of Bs-RNase III activity on uniformly labeled scRNA, in B. subtilis protein extracts. Control lane, C, no extract added. Time (in minutes) of incubation with rncS⁺ extract is indicated at the top of each lane. Lane marked ‘rncS’ indicates incubation for 12 min with extract prepared from strain that is deleted for the rncS gene. Schematics of scRNA products are on the left. Size markers (nucleotides) are on the right. (B) Quantitation of percent of full-length scRNA (solid circles), scRNA cleaved at A site (squares) and scRNA cleaved at A and b sites (triangles).