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Design of the conditional PLtetO-1 orn mutant. The PLtetO-1 promoter together with a cassette coding for the kanamycin resistance marker (KmR) and the Tet-repressor (TetR) were inserted directly in front of orn. The arrows correspond to the direction of transcription. DH5aZ1 (19) was the source of TetR and terminator T1, and plasmid pZE21-MCS1 (19) was the source of the PLtetO-1, kmR and terminator T0.
