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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Feb 6;1771(4):558–563. doi: 10.1016/j.bbalip.2007.01.016

Fig. 5. Incorporation of AA and DHA into phospholipid esters.

Fig. 5

Replicate dishes of PC12 cells were incubated for 3 hrs with 0.1 μCi 3H-AA or 14C-DHA and with 10 μM of the corresponding nonradioactive fatty acids. Phospholipids were extracted, separated by TLC and the radioactivity in individual spots was measured. Quantitites of PC, PS, PI and PE were determined by phosphate analysis. Data are the means ± SEM of 5 dishes and are expressed as dpm 3H or 14C incorporated/nmol PO4. ***, P<0.001 for differences between AA and DHA-treated groups.