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Indirect immunofluorescence of swim-up, permeabilized human sperm, including immunofluorescence staining for meichroacidin (A and C) and phase contrast pictures (B and D). The sperm were permeabilized with 0.1% saponin before probing with serum. The immune serum (A) stained the entire sperm tail including middle piece, principal piece and end piece. No immunostaining was observed with preimmune serum (C).
