Fig. 7.

Indirect immmunofluorescent staining of sections of human testis (A and B), trachea (C-F) spinal cord (G-J) and liver (K and L). Sections were probed with either rat anti-meichroacidn sera (B, D and H) or pre-immune sera (A, C and G) and prepared for indirect immunofluorescence staining. The same sections were counterstained with DAPI II for nuclear staining. The antibody mainly stained the tails of the testicular spermatozoa (ts) in the lumen (L) of the seminiferous tubule. In the trachea, a region at the luminal surface of the epithelium corresponding to the location of ciliary structures was stained by the antibody (D and F). Similarly the cilia of the ependymal epithelium lining the central canal of the spinal cord were stained with the antibody (H and J). Figures E and I represent phase contrast images showing magnified views of a portion of the ciliated epithelium of the trachea and ependyma respectively, F and J depict the corresponding images stained with the RSP44 antibody showing staining of the cilia (CL). The liver section did not show any immunoreactivity when stained with the immune serum (L) and was comparable to the pre-immune control (K).