Figure 5.

Strong correlation between mRNA deadenylation and translational repression. (A) Time-course analysis of luciferase synthesis. Capped and polyadenylated FLuc-6xT mRNA and capped RLuc mRNA were translated. Aliquots were taken for luciferase assays at time points 0, 20, 30, 40, and 60 min from the start of translation. (B) In parallel with the analyses of luciferase activities, RNAs were extracted from the aliquots taken at each time point, and were subjected to Northern hybridization analyses using the FLuc probe.