Skip to main content
. 2007 Aug 1;21(15):1937–1948. doi: 10.1101/gad.1553607

Figure 3.

Figure 3.

The mitochondria-targeted oligomerization domain of Bax is capable of causing apoptosis in the DKO cells. (A) Gel-filtration analysis of the mitochondria-targeted oligomerization domain of Bax and its mutants in the DKO cells. Plasmids for the indicated Bax wild type and mutants were transfected individually with or without the tBid expression plasmid into the DKO cells. (B) Apoptotic activities of the mitochondria-targeted Bax oligomerization domain and its mutants in the DKO cells. The indicated expression plasmids were cotransfected with a GFP expression plasmid (pEGFPC3) into DKO cells. Twenty hours after transfection, cells were stained with Hoechst dye. The percentage of GFP-positive cells that underwent nuclear condensation and fragmentation were quantified. The results are the average of at least three independent transfections. (C) Summary of the locations of the oligomerization domain and the apoptosis domain identified in the present study. The rectangles represent α-helices of Bax. The green color indicates the domains identified in this study.