Fig. 8.
Expression of UCP1, -2, and -3. (A and B) Representative Northern blots of total RNA (5-6 μg) prepared from the skeletal muscles, white adipose tissue, heart, liver, and brown adipose tissue of WT and Acc2-/- mutant mice. The filter was probed several times (after stripping) with the 32P-labeled cDNA fragments of UCP1, -2, and -3 and 18S ribosomal RNA (for quantification analysis). (C) Comparison of mRNA levels in different mouse tissues. B, brown adipose; L, liver; H, heart; M, skeletal muscle; W, white adipose. Data represent three Northern blots from WT and mutant mice that were hybridized with each UCP and 18S ribosomal RNA probe. The intensity of the band corresponds to each UCP normalized to the values of 18S mRNA signals. Values are mean ± SD.