Fig. 3.
Association between p57Kip2 and Skp2 in HeLa cells. (A) Cells were incubated with 100 nM DEX for 8 h and then with 10 μM MG132 for 6 h, after which cell lysates were subjected to IP with either rabbit Abs to p57Kip2 or control rabbit IgG. The resulting precipitates were subjected to immunoblot analysis with Abs to p57Kip2 or Skp2, as indicated. (B) After transfection (24 h) with expression vectors for the indicated proteins, cells were incubated for 6 h with MG132 (10 μM). Cell lysates were then subjected to IP with Abs to Myc. The immunoprecipitated proteins were subjected to immunoblot analysis with abs to Myc or HA.