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. 1998 Mar 3;95(5):2400–2405. doi: 10.1073/pnas.95.5.2400

Figure 2.

Figure 2

Induction of the de novo appearance of [PSI+] in [psi−][PIN+]74-D694 transformants with SUP35- and SUP45-bearing plasmids. (A) Spots show the growth of cotransformants with the plasmid pairs indicated on galactose (SGal-Ura,Leu,His) and on glucose (SC-Ura,Leu,His) media where GAL∷SUP35 is induced or repressed, respectively, and on repressing SC-Ade medium for suppression analysis. Arrows indicate replica plating. Growth on SC-Ade following the induction of GAL∷SUP35 is indicative of [PSI+]. Growth of transformants bearing the pJDB207 control vector (not shown) was essentially the same as the growth of YEp13-bearing transformants. (B) Spots show the growth of cotransformants on the media listed. Growth on SC-Ade in lanes A–F follows replica plating from SC-Ura,Leu,His containing 0, 10, 20, 50, 100, and 200 nM β-estradiol, respectively. +, Presence of the SUP45- and SUP35-bearing plasmids pJDB207-SUP45 and pGAL∷SUP35, respectively; −, presence of control vectors not bearing SUP45 or SUP35, pJDB207 and YCp50, respectively. Plasmid pHCA/GAL4(1–93).ER.VP16 was also present in all cotransformants. pJDB207-SUP45 caused severe growth reduction on adenineless media following induction of the GAL∷SUP35 construct by galactose regardless of the presence of the pHCA/GAL4(1–93).ER.VP16 plasmid (data not shown).