Figure 2.

Chromosomal localization of SHOT and OG-12. (A) Cohybridization of biotinylated human PAC 83I04 (yellow) and digoxygenin-labeled chromosome 3 specific painting probe. Chromosomes 3 are displayed separately as gray scale image demonstrating the 4′,6-diamidino-2-phenylindole bands and as full-color image showing the specific hybridization signals. (B) Hybridization of the biotinylated B212cos/17 kb EcoRI fragment, containing the entire OG-12 gene to mouse metaphase chromosomes. Metaphase spreads were prepared from methotrexate-synchronized and BrdUrd (BrdU)-treated mouse spleen cultures as previously described (19). The hybridized probe was detected using avidin-fluorescein isothiocyanate (Vector Laboratories) and is pseudocolored in yellow. The chromosomes are counterstained with propidium iodide and pseudocolored in red. The probe hybridized to chromosome 3, band E3-F1. Ideogram of mouse chromosome, representing schematically the G-banding pattern. The two homologues of mouse chromosome 3 are enlarged and displayed next to the ideogram.