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Western analyses of Ian4 protein. (a) Thymocyte (Thy) and lymph node cell (LNC) lysates from Ian4+/+ BBDR and WF and Ian4-/- BBDP rats. (b) Lymph node T cell, splenic B cell, and macrophage lysates from Ian4+/+ BBDR and WF.ART2a (WF*, see ref. 9) rats. (c) Purified mitochondrial lysates from T cells and thymocytes of Ian4+/+ BBDR and WF and Ian4-/- BBDP rats. Actin and cytochrome c were used as loading controls.
