Proteomic analyses. Purified mitochondrial proteins from
Ian4+/+ WF and Ian4-/- BBDP rat T
cells resolved by SDS/PAGE. Bands 1, 2, and 5 were differentially expressed in
Ian4+/+ vs. Ian4-/- T cells, excised,
and identified by MALDI-TOF mass spectrometry. Not all differentially
expressed bands were examined. Bands 3 and 4, which were similarly expressed,
were excised to verify the purity of the mitochondrial isolation. Identities
and gene identifiers for excised bands are indicated. Shown is one of two
similar gels.