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Selective, sensitive dot-blot assay for assembled forms of soluble Aβ1-42. (A) Immunoblot of ADDL preparations (100 fmol) shows that M93 antibody selectively identifies oligomers (Right), whereas R165 antibody identifies only monomer (Left). (B) A dot blot immunoassay using M93 detects ADDLs with a sensitivity of 1 fmol, but detects monomers only at levels 1,000-fold higher.
