Table 3. Inhibition of ZEB1 by RNAi.
Fold difference vs. mock-transfected cells
|
||
---|---|---|
Treatment | ZEB1 mRNA | E-cadherin mRNA |
Mock | 1.0 | 1.0 |
ZEB-188 | −6.5 | 2.2 |
Mock + lithium | 1.0 | 15 |
ZEB-188 + lithium | −4.0 | 23 |
Mock + TSA | −2.3 | 64 |
ZEB-188 + TSA | −7.0 | 223 |
H661 cells were mock-transfected, transfected with the indicated siRNA as described in the text, or treated with siRNA and either TSA or Li+. The Ct values were normalized to GAPDH, and the fold induction was calculated as in Table 1.