Commitment of primitive cells with cardiac hypertrophy. (A and
B; the same field) A cardiac progenitor (A, arrowhead) shows
c-kit on the surface membrane (green) and GATA-4 (A, red) in the
nucleus, and the myocyte progenitor (B, arrow) exhibits in its
nucleus GATA-4 (A, red) and MEF2 (B, white). (C and
D; the same field) A cardiac progenitor (C, arrowhead) shows
MDR1 on the surface membrane (purple) and GATA-4 (C, red) in the
nucleus, and a myocyte progenitor (D, arrow) exhibits in its nucleus
GATA-4 (C, red) and MEF2 (D, white). (E, F, and
G) Myocyte precursors (arrows) show on the surface membrane c-kit
(E, green), MDR1 (F, purple), or Sca-1-like (G,
yellow). A thin cytoplasmic layer is positive for cardiac myosin
(E-G, red) and nuclei express MEF2 (E-G, white).
(Bars = 10 μm.)