Table 2. Plasmids used for complementation assays.

Plasmid	Construction	Relevant protein products	Ref.
pKM101	Natural IncN plasmid	All pKM101 proteins	18
pMTX681	pSU19::oriT, traK, traI	pKM101 TraK and TraI	This work
pSU1092	ω insertion in trwA	All R388 proteins except TrwA	19
pSU1404	pSU4051(MobW)::Tn5tac1 in trwB	R388 TrwA and TrwC	20
pSU1423	pSU18::mobW	R388 TrwA, TrwB, and TrwC	19
pSU1425	R388 without EcoRI site	All R388 proteins	20
pSU1443	pSU1425::Tn5tac1 in trwB	All R388 proteins except TrwB	21
pSU1445	pSU1425::Tn5tac1 in trwC	All R388 proteins except TrwC	21
pSU2007	R388 KmR	All R388 proteins	22
pSU4132	pSU1425::Tn5tac1 in trwD	All R388 proteins except TrwD	23
pSU4133	pSU1425::Tn5tac1 in trwK	All R388 proteins except TrwK	This work
pSU4134	pSU1425::Tn5tac1 in trwE	All R388 proteins except TrwE	This work
pSU4280	pSU19::mobN	pKM101 TraK, TraJ, and TraI	This work
R6K-drd	Derepressed R6K plasmid	All R6K transfer proteins	24